Journal: Frontiers in Immunology

Article Title: USP50 regulates NLRP3 inflammasome activation in duodenogastric reflux-induced gastric tumorigenesis

doi: 10.3389/fimmu.2024.1326137

Figure Lengend Snippet: USP50 stimulates HMGB1 release in DGR-induced gastric inflammation. (A, B) mRNA levels of HMGB1, HSP70, HSP90, S100A8, and S100A9 were detected via qRT-PCR in U937 cells (A) or RAW264.7 cells (B) treated with DMSO or TDCA (200μM, 24 h). (C-H) ELISA was used to measure the concentrations of HMGB1 (C) , HSP70 (D) , HSP90 (E) , S100A8 (F) , and S100A9 (G) in the supernatant of U937 cells and RAW264.7 cells, while ATP concentration (H) was surveyed by ATP assay kit. (I) The protein level of HMGB1 was detected in gastric tissues of Sham and GJ mice by western blotting. Image J was used for quantitative analysis, with GAPDH as loading control. (J) The level of HMGB1 between normal gastric tissues and gastric cancer tissues was analyzed via GEPIA database (gepia.cancer-pku.cn/). (K) The IHC staining of HMGB1 was performed with sections from BRG patients and GC patients. (L) IRS was calculated for (K) . (M, N) HMGB1 was assayed by ELISA in the supernatant of U937 cells with USP50 overexpression (M) or knock-down (N) . * P < 0.05, ** P < 0.01, and *** P <0.001. "ns" means "no significance" ( P >0.05).

Article Snippet: Human S100A8 (E-EL-H1289c), Human S100A9 (E-EL-H1290c), HSP90 ELISA Kit (E-EL-H1864c), Human HMGB-1 ELISA Kit (E-EL-H1554c), Mouse HMGB-1 ELISA Kit (E-EL-M0676c) were obtained from Elabscience Biotechnology Co., Ltd, China.

Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Concentration Assay, ATP Assay, Western Blot, Control, Immunohistochemistry, Over Expression, Knockdown

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 1 Representative photomicrographs of myocardium tissues. A, tumor cells (arrow) were observed in the myocardial fibers of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 3 Representative photomicrographs of kidney tissues. A, tumor cells (arrow) were observed in the kidney tissues of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: Journal of Integrative Agriculture

Article Title: Study on Hsp90 Expression in Different Tissues and Its Antibody in Serum of Chickens Infected with Marek's Diseases

doi: 10.1016/s2095-3119(13)60514-6

Figure Lengend Snippet: Fig. 2 Representative photomicrographs of liver tissues. A, tumor cells (arrow) were observed in the liver tissues of chickens infected with MD (H.E. staining). B, positive immunolabelling of Hsp90 (arrow) was localized mainly in the cytoplasm of tumor cells (IHC staining). C, Hsp90 positive signals in negative control chickens (IHC staining).

Article Snippet: The Hsp90 concentration in the tissues was determined according to the manufacturer’s protocol (Cat. no. CSBE12873c, Chicken heat shock protein 90 (Hsp90) ELISA Kit, Cusabio Biotech Co., Ltd., China).

Techniques: Infection, Staining, Immunohistochemistry, Negative Control

Journal: PLoS ONE

Article Title: Biological Responses of Three-Dimensional Cultured Fibroblasts by Sustained Compressive Loading Include Apoptosis and Survival Activity

doi: 10.1371/journal.pone.0104676

Figure Lengend Snippet: Fibroblasts were seeded to collagen sponge and incubated for 24(□) or 200 mmHg (▪) compression for 6 h. Total mRNA was extracted after WST-1 assay, and mRNA expression was assessed using real-time RT-PCR. The expression of the target genes in the 6 h–200 mmHg group relative to the value in the 6 h–0 mmHg group was calculated by the comparative Ct method using the 18S ribosomal RNA gene as an internal control. The results are represented as the mean ± SEM (error bars) of five experiments. Statistical analysis was performed using the Student’s t test between the 0 mmHg group and the 200 mmHg group, and statistical significance was taken as p <0.05. A value of p was expressed as: *; p <0.05, **; p <0.01, and ***; p <0.001. A: The transcription factors of various Hsps . B: various Hsps C: Bcl2 is an antiapoptotic gene, and Bax is a proapoptotic gene. D and E: Immunostaining for HSP90α. Representative sections of (D) the 6 h–0 mmHg group and (E) the 6 h–200 mmHg group. Higher expression and nucleus translocation of HSP90α was observed in the 200 mmHg group (E) when compared with the 0 mmHg group (D). Scale bars = 20 µm for all images.

Article Snippet: HSP90α concentration was measured using the Rat Heat Shock Protein 90α ELISA kit (CUSABIO BIOTECH, Wuhan, China).

Techniques: Incubation, WST-1 Assay, Expressing, Quantitative RT-PCR, Control, Immunostaining, Translocation Assay

Journal: PLoS ONE

Article Title: Biological Responses of Three-Dimensional Cultured Fibroblasts by Sustained Compressive Loading Include Apoptosis and Survival Activity

doi: 10.1371/journal.pone.0104676

Figure Lengend Snippet: Fibroblasts were seeded to collagen sponge and incubated for 24(A: HSP90α, B: HA, C: PGE 2 ) was measured by ELISA. A value of concentration was normalized by WST-1 value. The results are represented as the mean ± SEM (error bars) of five experiments. Statistical analysis was performed using the Dunnett’s multiple test between non-loaded group and each of loaded group, and statistical significance was taken as p <0.05. A value of p was expressed as: *; p <0.05, **; p <0.01, and ***; p <0.001.

Article Snippet: HSP90α concentration was measured using the Rat Heat Shock Protein 90α ELISA kit (CUSABIO BIOTECH, Wuhan, China).

Techniques: Incubation, Enzyme-linked Immunosorbent Assay, Concentration Assay

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel method for the establishment of autologous skin cell suspensions: characterisation of cellular sub-populations, epidermal stem cell content and wound response-enhancing biological properties

doi: 10.3389/fbioe.2024.1386896

Figure Lengend Snippet: Proteomic analysis of the ACS secretome. Semiquantitative analysis using proteome arrays and quantitative detection by ELISA assays of soluble mediators secreted by ACS-derived cell populations cultured in serum-free medium at the indicated time points. Supernatants from cultures established from ACS were collected and analysed as detailed in the Methods. (A) Relative intensity values of densitometric analysis of ACS-secreted soluble factors using an angiogenesis array on days 1, 3 and 6 after isolation. (B) Relative intensity values of similar analysis using a cytokine proteome array on the same time-points post-isolation. (C) Detection of Interleukin-1 alpha, HMGB1 and Hsp90α in the ACS secretome by analyte-specific ELISA. *, p < 0.05; **, p < 0.01; ***, p < 0.001; ****, p < 0.0001; ns, non-significance.

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was used for quantitative determination of IL-1α (#DLA50, R&D Systems, Bio-Techne), HMGB1 (#NBP2-62766, Novus Biologicals, supplied by Bio-Techne) and HSP90α (#NBP2-76448, Novus Biologicals) in ACS conditioned medium.

Techniques: Enzyme-linked Immunosorbent Assay, Derivative Assay, Cell Culture, Isolation

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: A novel method for the establishment of autologous skin cell suspensions: characterisation of cellular sub-populations, epidermal stem cell content and wound response-enhancing biological properties

doi: 10.3389/fbioe.2024.1386896

Figure Lengend Snippet: Schematic summary of proposed positive effects of ACS application onto a wound. ACS isolates contain the main skin cell sub-types, such as keratinocytes and fibroblasts (melanocytes are not indicated), as well as non-viable cells which may release DAMPs, such as HSP90α and HMGB1. It is postulated that the rapidly proliferating keratinocytes would be able to “take” into the wound bed (A) and thus directly contribute to re-epithelialisation. Keratinocyte activation and migration at the ‘wound edge’ (B) would be enhanced by the growth factors, cytokines and DAMPs secreted by cells contained in the ACS. Fibroblast migration into the wound bed (C) would also be stimulated by the secreted EGF and HSP90α in order to facilitate HDF-mediated regeneration of the appropriate supporting ECM. The diagram was created using BioRender ( www.biorender.com ).

Article Snippet: Enzyme-linked immunosorbent assay (ELISA) was used for quantitative determination of IL-1α (#DLA50, R&D Systems, Bio-Techne), HMGB1 (#NBP2-62766, Novus Biologicals, supplied by Bio-Techne) and HSP90α (#NBP2-76448, Novus Biologicals) in ACS conditioned medium.

Techniques: Activation Assay, Migration

Journal: PLoS ONE

Article Title: Key role of Extracellular RNA in hypoxic stress induced myocardial injury

doi: 10.1371/journal.pone.0260835

Figure Lengend Snippet: Level of SI markers in plasma was analysed after exposure to hypoxia for different time durations (0–24 hrs); (a) eDNA (μg/ml), (b) eRNA (μg/ml), (c) HMGB1 (ng/ml), (d) vWF (ng/ml), (e) HSP70 (ng/ml), (f) HSP90 (ng/ml), and (g) s100b (pg/ml). Data are shown as mean ± SEM (n = 5/group/each time point) from one experiment representative of three independent experiments, all performed in triplicate. One-way ANOVA revealed statistical significance in the results (*p< 0.05).

Article Snippet: Quantitative ELISA kits were procured as follows: Mouse HMGB1 protein ELISA kit (MBS722248) and Mouse vWF ELISA kit (MBS260427) from MyBiosource Inc (CA, USA); HSP70 high sensitivity ELISA kit (ADI-EKS-715) from Enzo Life Sciences (NY, USA); Mouse HSP90 ELISA kit (NBP2-76449) from Novus Biologicals (CO, USA); Mouse S100B ELISA kit (E-EL-M1033) from Elabscience (TX, USA).

Techniques: Clinical Proteomics

Journal: Frontiers in Oncology

Article Title: Evaluation of colorectal cancer liver metastases based on liquid biopsy combined with folate receptor– Positive circulating tumor cells and HSP90

doi: 10.3389/fonc.2022.912016

Figure Lengend Snippet: Comparisons of parameters between patients with colorectal cancer and liver and non-liver metastases.

Article Snippet: Plasma HSP90 levels were detected by HSP90 protein ELISA kit (Antai Protegen Biotechnology Development Co., Ltd., Antai, China).

Techniques:

Journal: Frontiers in Oncology

Article Title: Evaluation of colorectal cancer liver metastases based on liquid biopsy combined with folate receptor– Positive circulating tumor cells and HSP90

doi: 10.3389/fonc.2022.912016

Figure Lengend Snippet: Analysis of clinical data of cohorts: (A–D) Comparison of the serology of folate receptor plus circulating tumor cells (FR+CTC), heat shock protein 90 (HSP90), CEA, and CA199 levels between colorectal cancer patients with and without liver metastasis. The data are presented as mean ± standard error of the mean (SEM). Heatmap expression matrix of serological marker correlations in non-metastatic (E) and metastatic colorectal cancers (F) . “*” Represents the p-value of the two groups after statistical test, * represents p<0.05, ** represents p<0.01, *** represents p<0.001, and **** represents p<0.0001.

Article Snippet: Plasma HSP90 levels were detected by HSP90 protein ELISA kit (Antai Protegen Biotechnology Development Co., Ltd., Antai, China).

Techniques: Comparison, Expressing, Marker

Journal: Frontiers in Oncology

Article Title: Evaluation of colorectal cancer liver metastases based on liquid biopsy combined with folate receptor– Positive circulating tumor cells and HSP90

doi: 10.3389/fonc.2022.912016

Figure Lengend Snippet: Diagnostic and prognostic capabilities of folate receptor plus circulating tumor cells (FR+CTC) and heat shock protein 90 (HSP90). (A) Receiver operating characteristic (ROC) curve analysis: the diagnostic ability of single FR+CTC, HSP90, CEA, and CA199 in differentiating liver metastatic colorectal cancer. (B) ROC curve analysis: the diagnostic ability of combined markers in differentiating liver metastatic colorectal cancer were FR+CTC and HSP90, FR+CTC and CEA, and HSP90 and CEA, respectively. High expression of FR+CTC (C) and HSP90 (D) predicts the median disease-free survival (mDFS) of liver metastatic colorectal cancer.

Article Snippet: Plasma HSP90 levels were detected by HSP90 protein ELISA kit (Antai Protegen Biotechnology Development Co., Ltd., Antai, China).

Techniques: Diagnostic Assay, Expressing

Journal: Frontiers in Oncology

Article Title: Evaluation of colorectal cancer liver metastases based on liquid biopsy combined with folate receptor– Positive circulating tumor cells and HSP90

doi: 10.3389/fonc.2022.912016

Figure Lengend Snippet: Diagnostic value of alone and combined biomarkers for distinguishing patients with colorectal cancer liver metastases (LMCRC).

Article Snippet: Plasma HSP90 levels were detected by HSP90 protein ELISA kit (Antai Protegen Biotechnology Development Co., Ltd., Antai, China).

Techniques: Diagnostic Assay

Journal: Frontiers in Oncology

Article Title: Evaluation of colorectal cancer liver metastases based on liquid biopsy combined with folate receptor– Positive circulating tumor cells and HSP90

doi: 10.3389/fonc.2022.912016

Figure Lengend Snippet: Establishing a logistic regression risk assessment model. Univariate (A) and multivariate (B) analyses of risk factors for liver metastasis of colorectal cancer. (C) Receiver operating characteristic (ROC) curve of risk scoring model. (D) Risk score model and survival scatter plot of patients with colorectal cancer. High risk of folate receptor plus circulating tumor cells (FR+CTC) (C) and heat shock protein 90 (HSP90) (D) predicts the median disease-free survival (mDFS) of liver metastatic colorectal cancer. (E) High- and low-risk scores predict mDFS in patients with colorectal cancer liver metastases. (F) Comparison of diagnostic ability between risk model and single index.

Article Snippet: Plasma HSP90 levels were detected by HSP90 protein ELISA kit (Antai Protegen Biotechnology Development Co., Ltd., Antai, China).

Techniques: Comparison, Diagnostic Assay